Isolation and identification of Candida spp. from immunocompromised patients

Abstract Background Candida species is considered one of the normal inhabitant commensal microbiota human body. However, it can also act as an opportunistic pathogen especially in hospitals (nosocomial infection) and among immunocompromised patients. The accurate, precise, rapid reliable identification to level great importance for control management candidiasis. Results One hundred eighteen different samples were collected (59 urine samples, 39 oral swabs, 5 vaginal swabs 15 skin swabs) from patients (diabetics—pregnant women—patients underwent organ transplantation—cancer patients—burned wounded patients) probable existence species. Eighty-six out 118 (72.8%) typed macroscopically microscopically found be Upon streaking 86 isolates on CHROMagar plates separately, 48 gave green colonies, 25 rose 10 white 2 pale coloured colonies 1 isolate blue colonies. Forty-eight showed positive Germ tube test. API 20C assay was performed some with results similar those CHROMagar. performing PCR 14 using ITS1 ITS4 primers, 8 product bands appeared between 510 535 bp this difficult differentiate them ( C. albicans , tropicalis, krusei parapsilosis ). Five products at 871 glabrata ) last negative no band appeared. Further molecular studies should recommended fully very close bands. Antifungal activity (expressed by inhibitory zone) zinc oxide nanoparticles comparison commercially available antifungals (nystatin Voriconazole) carried upon obtained isolates. Zinc 100 μg/disc 9, 26, 40, 42 mm zones glabrata, respectively. only antifungal against krusei. Conclusions A sheet profile include morphotyping, biotyping genotyping reach a rapid, accurate diagnosis. causes myriad infections causing non-invasive, mucocutaneous severe systemic deep-seated disease. Repress growth ZnONPs provides insight towards their therapeutic application prevention -associated infections. effect combined medicines maybe recommended.